Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight stimulation | Bos taurus | |
Endogen lipids | soluble in CHCl3/CH3OH, 2:1, activation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak | Bos taurus | |
Cu2+ | strong | Bos taurus | |
Mn2+ | - |
Bos taurus | |
additional information | not inhibited by amphomycin; not inhibited by EDTA | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Bos taurus | 16020 | - |
microsome | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of divalent cations | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDPmannose + oligosaccharide-lipid acceptor | Bos taurus | involved in biosynthesis of lipid-linked oligosaccharides up to (Man)5-(GlcNAc)2, involved in the production of two 1,2-linked mannosyl residues in mammalian lipid-linked tetradecasaccharide of the structure (Glc)3(Man)9(GlcNAc)2, the major precursor for eukaryotic en-bloc-glycosylation of asparagin-linked glycoproteins | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
cow | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | lactating mammary tissue | Bos taurus | - |
Storage Stability | Organism |
---|---|
4°C, solubilized enzyme, 2 weeks: about 40% loss of activity, 7 days: about 20% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + lipid-linked Manalpha(1-3)Manbeta-GlcNAcbeta-GlcNAc | reaction via penta- and hexasaccharide intermediates to a lipid-linked heptasaccharide, presumably multiple mannosyltransferases involved | Bos taurus | GDP + lipid-linked Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta-GlcNAcbeta-GlcNAc | - |
? | |
GDP-mannose + oligosaccharide-lipid acceptor | acceptors can be mannose or Man-(GlcNAc)2 to (Man)5-(GlcNAc)2, lipid-linked tetrasaccharide Manalpha(1-3)Manbeta-GlcNAcbeta-GlcNAc, presumably multiple mannosyltransferases involved | Bos taurus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? | |
GDP-mannose + oligosaccharide-lipid acceptor | acceptor isolated from baby hamster kidney cells, i.e. BHK cells | Bos taurus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? | |
GDPmannose + oligosaccharide-lipid acceptor | involved in biosynthesis of lipid-linked oligosaccharides up to (Man)5-(GlcNAc)2, involved in the production of two 1,2-linked mannosyl residues in mammalian lipid-linked tetradecasaccharide of the structure (Glc)3(Man)9(GlcNAc)2, the major precursor for eukaryotic en-bloc-glycosylation of asparagin-linked glycoproteins | Bos taurus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? | |
additional information | no sugar donor: mannosylphosphorylretinol | Bos taurus | additional information | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.6 | broad | Bos taurus |